不明原因不育男性精子顶体酶活性与精子膜表面抗体检测分析

Objective To investigate the relationship between sperm acrosin activity and antibody coating of spermatozoa in unexplained infertile males. Methods A modified method of Kennedy assay was used to detect the sperm acrosin activity of 214 men with unexplained infertility , and antibody coating of spermatozoa was examined by the mixed antiglobulin reaction (MAR) test, then analyze the correlation between the two indexes. Results In the 214 infertile males, 158 people had normal sperm acrosin activity (normal group), while 56 people had abnormal sperm acrosin activity (abnormal group). The level of antibody coating of spermatozoa in abnormal group was significantly higher than that in normal group (p < 0.05). The sperm acrosin activity was significantly negatively related with the level of antibody coating of spermatozoa in unexplained infertile males (rs = - 0.184, p < 0.05). Conclutions The lower sperm acrosin activity may be one of the reasons for unexplained male infertility. And the antibody coating of spermatozoa has certain effects on sperm acrosin activity.