细胞分裂素（6-BA）对缺磷白羽扇豆的排根形成和有机酸分泌的影响
Effects of 6-BA on Cluster Root Formation and Organic Acids Exudation in White Lupin grown under Phosphrous Deficiency
缺磷条件下白羽扇豆能够形成排根，并增加有机酸的分泌。但上述过程的调节机制尚不清楚。本文研究了6-BA（6-苄基腺嘌呤）处理对缺磷的白羽扇豆排根形成的影响，并利用非破坏性原位收集方法和高效液相色谱（HPLC）技术研究了6-BA处理对根簇分泌柠檬酸和苹果酸及组织中这两种有机酸含量的影响。结果表明，6-BA处理不影响植株的生长和磷素在体内的分配，但明显抑制了缺磷白羽扇豆的根簇形成和有机酸分泌。其中，经低浓度6-BA（10-8 mol/L）处理后转移至不含6-BA的缺磷营养液中继续生长的植株，其根簇形成和有机酸分泌得到恢复，甚至新形成的根簇数目及有机酸分泌量多于未经6-BA处理的缺磷植株；但高浓度6-BA（10-7 mol/L）对根簇形成和有机酸分泌表现出不可恢复性抑制。6-BA处理也在一定程度上影响了根簇组织中的有机酸含量。对6-BA影响缺磷的白羽扇豆排根形成和有机酸分泌的可能机制进行了讨论。
hosphorus (P) deficiency results in cluster root formation and increased organic acid exudation. The regulatory mechanisms for these processes, however, are still not clear. In the present study, influences of 6-BA (6-benzyl aminopurine) on cluster root formation, exudation of citrate and malate and their concentration in the root clusters of P-deficient white lupin plants were studied by using of non-destructive localized collection method and high-performance liquid chromatography (HPLC) analyze technique. The results showed that application of exogenous 6-BA in nutrient solution did not influence plant growth and P distribution within plant, compared with the P-deficient plants. However, the cluster root formation and organic acid exudation were inhibited. These inhibitory effects could be recovered after omitting P from the growth medium, and even some stimulatory effects was observed, when lower concentration of 6-BA (10-8 mol/L) was applied. While the inhibitory effects was not
李春俭、梁瑞霞、宋建兰
植物学生物化学
缺磷白羽扇豆排根有机酸分泌6-BA（6-苄基腺嘌呤）
Phosphorus deficiencywhite lupincluster rootsorganic acid exudation6-benzyl aminopurine
李春俭,梁瑞霞,宋建兰.细胞分裂素（6-BA）对缺磷白羽扇豆的排根形成和有机酸分泌的影响[EB/OL].(2004-04-14)[2025-08-17].http://www.paper.edu.cn/releasepaper/content/200404-49.点此复制
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