推拿对坐骨神经损伤大鼠TrkA的影响

Objective Observe the influence of Tuina therapy to TrkA of sciatic nerve injury model rats. Probe the biological mechanisms of Tuina therapy in sciatic nerve injury. Method Use the nerve clamping method to make the sciatic nerve injury model. Observe the oblique board test and thermal withdraw latency test in blank control group, sham-operated group, model control group, sham-Tuina group and Tuina group as behavioral evidences. Observe TrkA protein by using immunohistochemistry. Result The oblique board test scores of the model group and sham-Tuina group are significantly lower than blank control group, while Tuina group score is much higher. The thermal withdraw latency test scores of the model group and sham-Tuina group are significantly higher than blank control group, while Tuina group score is much lower. The immunohistochemistry shows that the amount of the TrkA proteins are all significantly different in model control group, sham-Tuina group and Tuina group. TrkA are significantly higher in Tuina group. Conclusion Tuina therapy treats the sciatic nerve injury by increasing TrkA. As a result, the survival mechanism of nerve cell is promoted by Tuina therapy, so the feeling function and motor function of the rats are improved.