肝移植术后稳定生存者外周血中差异表达miRNA及其功能预 测
目的 寻找肝移植术后长期稳定生存者外周血中有意义的生物学标志物。方法 纳入肝移植术后稳定存活患者29例（STA组），反复排斥反应者10例（RJ组），健康对照者17例（HC组），利用芯片检测各组PBMCs中microRNAs的表达谱，并用Real-time PCR对差异表达的microRNAs进行验证。结果 芯片结果显示STA组对比于RJ组共13个microRNA表达明显下调，经Real-time PCR验证3个microRNA（miR-106b, miR-18b, miR-340）表达下调。结论 肝移植患者外周血miR-106b、miR-18b、miR-340可作为肝移植术后潜在标志物。
Objective To determine whether the miRNA expression profile in peripheral blood mononuclear cells (PBMCs) differs between liver transplant recipients with long-term stable survival and those with acute rejection. Methods Twenty-nine liver transplant recipients with long-term stable survival (STA) group, 10 recipients with acute rejection (RJ group), and 17 healthy subjects (control group) were recruited for genome-wide microarray analysis of miRNA expressions in the PBMCs. The differentially expressed miRNAs among the 3 groups were validated by real-time PCR, and the targets of these miRNAs were predicted. Results Compared with the RJ group, the STA group showed down-regulation of 13 miRNAs in the PBMCs. Of these down-regulated miRNAs, miRNA-18b, miRNA-340 and miRNA-106b were validated by real-time PCR, and the latter two miRNAs were predicted to target the TGF- pathway. Conclusions The differentially expressed miRNAs in liver transplant recipients with long-term stable survival, namely miRNA-18b, miRNA-340 and miRNA-106b, can be potential clinical biomarkers to predict the outcomes of liver transplantation.
张鹏、钟克波、何晓顺、朱晓峰
医学研究方法基础医学内科学
miRNA生物标志物肝移植免疫耐受排斥反应
张鹏,钟克波,何晓顺,朱晓峰.肝移植术后稳定生存者外周血中差异表达miRNA及其功能预 测[EB/OL].(2017-12-07)[2025-08-25].https://chinaxiv.org/abs/201712.00736.点此复制
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