Detection and characterization of circulating microvesicles containing Shiga toxin type 2 (Stx2) in a rat model of Hemolytic Uremic Syndrome
Detection and characterization of circulating microvesicles containing Shiga toxin type 2 (Stx2) in a rat model of Hemolytic Uremic Syndrome
Abstract Shiga toxin (Stx) producing Escherichia coli (STEC) are foodborne pathogens that release Stx and may develop Hemolytic Uremic Syndrome (HUS). Stx causes endothelial cell damage and leads to platelets deposition and thrombi formation within the microvasculature. It has been described that Stx activates blood cells and induces the shedding of proinflammatory and prothrombotic microvesicles (MVs) containing the toxin. In this sense, it has been postulated that MVs containing Stx2 (MVs-Stx2+) can contribute to the physiopathology of HUS, allowing Stx to reach the target organs and evading the immune system. In this work, we propose that circulating MVs-Stx2+ can be a potential biomarker for the diagnosis and prognosis of STEC infections and HUS progression. In this regard, we developed a rat HUS model by the intreperitoneal injection of a sublethal dose of Stx2 and observed: decrease in body weight, increase of creatinine and urea levels, decrease of creatinine clearance and histological renal damages. After characterization of renal damages we investigated circulating total MVs and MVs-Stx2+ by flow cytometry at different times after Stx2 injection. Additionally, we evaluated the correlation of biochemical parameters such as creatinine and urea in plasma with MVs-Stx2+. As a result, we found a significant circulation of Mvs-Stx2+ at 96 hours after Stx2 injection, nevertheless no correlation with creatinine and urea plasma levels were detected. Our results suggest that MVs-Stx2+ may be an additional biomarker for the characterization and diagnosis of HUS progression. Further analysis is required in order to validate MVs-Stx2+ as biomarker of the disease.
Moretton Marcela A.、Gomez Fernando、Ibarra Cristina、Jancic Carolina、Chiappetta Diego A.、Amaral Mar¨aa Marta、Sacerdoti Flavia
C¨￠tedra de Tecnolog¨aa Farmac¨|utica I, Facultad de Farmacia y Bioqu¨amica, Universidad de Buenos Aires||Universidad de Buenos Aires, Instituto de Tecnolog¨aa Farmac¨|utica y Biofarmacia (InTecFyB)||Consejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas (CONICET)Laboratorio de Fisiopatogenia, Departamento de Ciencias Fisiol¨?gicas, Instituto de Fisiolog¨aa y Biof¨asica (IFIBIO) Houssay, Facultad de Medicina, Universidad de Buenos AiresLaboratorio de Fisiopatogenia, Departamento de Ciencias Fisiol¨?gicas, Instituto de Fisiolog¨aa y Biof¨asica (IFIBIO) Houssay, Facultad de Medicina, Universidad de Buenos AiresInstituto de Medicina Experimental ¨C CONICET ¨C Academia Nacional de Medicina||Departamento de Microbiolog¨aa, Parasitolog¨aa e Inmunolog¨aa, Facultad de Medicina, Universidad de Buenos AiresC¨￠tedra de Tecnolog¨aa Farmac¨|utica I, Facultad de Farmacia y Bioqu¨amica, Universidad de Buenos Aires||Universidad de Buenos Aires, Instituto de Tecnolog¨aa Farmac¨|utica y Biofarmacia (InTecFyB)||Consejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas (CONICET)Laboratorio de Fisiopatogenia, Departamento de Ciencias Fisiol¨?gicas, Instituto de Fisiolog¨aa y Biof¨asica (IFIBIO) Houssay, Facultad de Medicina, Universidad de Buenos AiresLaboratorio de Fisiopatogenia, Departamento de Ciencias Fisiol¨?gicas, Instituto de Fisiolog¨aa y Biof¨asica (IFIBIO) Houssay, Facultad de Medicina, Universidad de Buenos Aires
基础医学微生物学生理学
Shiga toxinmicrovesiclescytotoxicityHUSSTEC
Moretton Marcela A.,Gomez Fernando,Ibarra Cristina,Jancic Carolina,Chiappetta Diego A.,Amaral Mar¨aa Marta,Sacerdoti Flavia.Detection and characterization of circulating microvesicles containing Shiga toxin type 2 (Stx2) in a rat model of Hemolytic Uremic Syndrome[EB/OL].(2025-03-28)[2025-06-21].https://www.biorxiv.org/content/10.1101/2022.02.17.480856.点此复制
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