基于薄膜电极的细胞电融合芯片及实验研究

microfluidic chip with discrete thin film microelectrode structure was developed for high efficiency cell electrofusion. The top/bottom thin film microelectrode array and the thin film microelectrodes patterned on sidewalls of the Durimide 7510 microchannel constructed the discrete "sandwich" microelectrode structures. This structure could generate nonuniform electric filed for dielectrophoresis-based cell alignment/pairing. The cavity between two adjacent microelectrodes was also filled by using the Durimide 7510. It overcame the shortcoming in traditional continuous protruding cell-electrofusion chip where many cells were trapped in the unfilled cavity. Cell alignment and electrofusion were performed on this chip by using K562 cells and good results were achieved. A low voltage pulses (~ 10 V) series could produce a electric filed strong enough for revisable electroporation. Most cells (~99%) were trapped on the surface of the thin film microelectrodes and almost no cells were docked between two adjacent electrodes on the same sidewall. More than 60% cells were aligned as cell-cell twins and about 40% cells were fused. Thus, compared with traditional cell electrofusion method and protruding microelectrode structures based method, higher alignment and fusion efficiency were achieved.and glass substrate and low depth-width ratio channel were helpful for the observation and control of the liposome fusion.