反复热性惊厥过程中CBS/H2S系统对HO-1/CO系统的作用
Hydrogen sulfide modulates carbon monoxide in recurrent febrile seizures in rats
目的 研究反复热性惊厥（febrile seizures，FS）过程中，内源性CBS/H2S系统对HO-1/CO系统的作用。方法 21日龄 SD大鼠，采用热水浴诱导大鼠惊厥，隔日 1 次，共 10 次。大鼠随机分为四组: 正常对照组、高热惊厥组、高热惊厥, FS + NaHS（硫化氢外源性供体）组，FS + HA（磷酸吡多醛-5`-磷酸-依赖性酶包括胱硫醚-β-合成酶抑制剂）组。采用分光光度计法测定大鼠血浆中CO含量；用原位杂交、免疫组化检测方法对海马HO-1 mRNA及蛋白表达进行定位及半定量分析。结果 硫化氢供体的干预可以增强HO-1/CO系统的表达，而磷酸吡多醛-5`-磷酸-依赖性酶包括胱硫醚-β-合成酶抑制剂可以抑制HO-1/CO系统的表达。结论 在反复热性惊厥过程中，气体信号分子H2S对HO-1/CO系统的表达具有重要的调节作用。
he aim of this study is to explore the effect of hydrogen sulfde on heme oxygenase-1/ carbon monoxide (HO-1/CO) system in recurrent febrile seizures (FS) in rats. On a rat model of recurrent FS, the measurement of plasma CO, the expressions of HO-1 were examined after treatment with NaHS, a donor of hydrogen sul?de, or hydroxylamine, an inhibitor of cystathionine b-synthase. The results found that sodium sulfhydrate (NaHS, a donor of hydrogen sul?de) increased the level of plasma CO, up-regulated the expression of HO-1. Hydroxylamine (an inhibitor of cystathionine b-synthase) decreased he level of plasma CO, down-regulated the expression of HO-1. These results suggest that H2S plays a regulatory role through modulating HO-1/CO system in the pathogenesis of recurrent FS.
韩颖、常杏芝、杨志仙、杜军保、秦炯
基础医学神经病学、精神病学生理学
惊厥发热性硫化氢一氧化碳
kSeizureFebrileHydrogen sul?deCarbon monoxide
韩颖,常杏芝,杨志仙,杜军保,秦炯.反复热性惊厥过程中CBS/H2S系统对HO-1/CO系统的作用[EB/OL].(2012-02-17)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201202-676.点此复制
评论