Identification of four linear B-cell epitopes on the SARS-CoV-2 spike protein able to elicit neutralizing antibodies
Identification of four linear B-cell epitopes on the SARS-CoV-2 spike protein able to elicit neutralizing antibodies
Abstract SARS-CoV-2 unprecedentedly threatens the public health at worldwide level. There is an urgent need to develop an effective vaccine within a highly accelerated time. Here, we present the most comprehensive S-protein-based linear B-cell epitope candidate list by combining epitopes predicted by eight widely-used immune-informatics methods with the epitopes curated from literature published between Feb 6, 2020 and July 10, 2020. We find four top prioritized linear B-cell epitopes in the hotspot regions of S protein can specifically bind with serum antibodies from horse, mouse, and monkey inoculated with different SARS-CoV-2 vaccine candidates or a patient recovering from COVID-19. The four linear B-cell epitopes can induce neutralizing antibodies against both pseudo and live SARS-CoV-2 virus in immunized wild-type BALB/c mice. This study suggests that the four linear B-cell epitopes are potentially important candidates for serological assay or vaccine development.
Wang Ying、Wang Yiting、Wang Jie、Li Lin、He Yufei、Han Xiaohan、Wen Hao、Wang Haoyu、Liu Guang、Sun Ting、Chen Yifan、Du Chen、Zhang Jing、Li Jing、Wang Hui、Yang Zeqian、Chen Shaolong、Liu Yong、Li Min、Li Wendong、Zhao Zhongpeng、Li Xinyang、Yang Xiaolan、Su Zhongyi、Wang Tiecheng、Wang Lu、Fan Yubo
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical SciencesKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical SciencesKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical SciencesKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical SciencesKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityState Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical SciencesKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityInstitute of Military Veterinary, Academy of Military Medical SciencesKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang UniversityKey Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University
基础医学生物科学研究方法、生物科学研究技术预防医学
linear B-cell epitopeSpike proteinSARS-CoV-2Neutralizing antibodyVaccine development
Wang Ying,Wang Yiting,Wang Jie,Li Lin,He Yufei,Han Xiaohan,Wen Hao,Wang Haoyu,Liu Guang,Sun Ting,Chen Yifan,Du Chen,Zhang Jing,Li Jing,Wang Hui,Yang Zeqian,Chen Shaolong,Liu Yong,Li Min,Li Wendong,Zhao Zhongpeng,Li Xinyang,Yang Xiaolan,Su Zhongyi,Wang Tiecheng,Wang Lu,Fan Yubo.Identification of four linear B-cell epitopes on the SARS-CoV-2 spike protein able to elicit neutralizing antibodies[EB/OL].(2025-03-28)[2025-06-15].https://www.biorxiv.org/content/10.1101/2020.12.13.422550.点此复制
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