棉花脂转运蛋白GHLTPⅡ12的克隆与分析
loning and analysis of cotton lipid transporter GHLTPⅡ12
培育优良棉花纤维品质的新品种是棉花育种的重要目标之一。目前，大量纤维发育相关的基因被挖掘，但是对其功能尚不明确。本研究在前期实验室研究的基础上，筛选出一个与纤维相关性较强的基因，并克隆该基因，命名GHLTPⅡ12。GHLTPⅡ12的cDNA全长为327bp，编码108个氨基酸，预测蛋白大小为11.595kD，理论等电点为8.87， 1-25处有信号肽，蛋白在53-108处有一个跨膜结构域，包含一个Hydrophobic seed 保守结构域，属于脂转运蛋白。定位于细胞质和细胞膜。
ultivating new varieties with excellent cotton fiber quality is one of the important objectives of cotton breeding. At present, a large number of genes related to fiber development have been excavated, but their functions are still unclear. On the basis of previous laboratory studies, this study screened out a gene with strong correlation with fiber, and cloned the gene, named GHLTPⅡ12. The full-length cDNA of GHLTPⅡ12 is 327bp, encoding 108 amino acids. the predicted protein size is 11.595kD, and the theoretical isoelectric point is 8.87. the protein has a transmembrane domain at 53-108, including a conserved domain of Hydrophobic seed, which belongs to lipid transporter. Localization in cytoplasm and cell membrane.
李会敏、陈丽婷、张子豪、鱼海鹏、李青、马丽梅、阎媛媛
植物学生物科学研究方法、生物科学研究技术农业科学技术发展
棉花基因克隆脂转运蛋白纤维发育
ottonGene cloningLipid transporterFiber development?????
李会敏,陈丽婷,张子豪,鱼海鹏,李青,马丽梅,阎媛媛.棉花脂转运蛋白GHLTPⅡ12的克隆与分析[EB/OL].(2021-05-31)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/202105-241.点此复制
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