逆转录病毒介导增强型绿色荧光蛋白基因在大鼠骨髓间充质干细胞中的表达1

ensity gradient centrifugation culture was conducted to obtain rat BMSCs which were then induced in the adipogenic direction. their surface antigen and the adipogenic-differentiation potential were detected by red oil O staining。On the basis of the above results,BMSCs were transfected with the recombinant retrovirus pLEGFP-N1 containing enhanced green fluorescent protein(EGFP)..The morphologic changes of the cells,the expression time and intensity of fluorescent light were observed,The transfection efficiency was detected.evaluate the possibility of labelling SD rat bone marrow stromal cells with green fluorescent protein so as to seeking an ideal cell tracing mark for cell transporting.Results as following, The morphology and purity of the rat BMSCs obtained were good.The adipocyte was significant after adipogenic induction.EGFP was successfully expressed after BMSCs were transfected with the retroviral vector pLEGFP-N1.The labeled cells were easy to observe within four weeks. We concluded that retrovirus is an ideal vector for its high transfection efficiency ,and long expression period of the fluorescent light.The technique of seeding cells labeled with fluorescent protein is good at monitoring the seeding cells.