清肺化痰、益气活血对肺纤维化大鼠不同时点TGF-β1mRNA表达的影响

Objective To study the effect of QFHT Principle，YQHX Principle， two method associated usages and two method to induct by stages on the model which idiopathic pulmonary fibrosis rat was formed by bleomycin injection via trachea.And explore the academic gist and functional mechanism of preventing the TGF-β1mRNA expression excreted by Alveolar Macrophage and Intercrop Macrophage.Methods Seventy-seven male SPF rate Wistar rats were randomly divided into 7 groups，11 in each group，i.e.the normal control group，the model group，the QFHT group，the YQHX group，the two method associated group，the two method to induct by stages group and the hydrocortisone group. Establishment of the cell model of Alveolar Macrophage and Intercrop Macrophage， and the cultured cells were stained with nucleic acid hybridization in situ to observe the TGF-β1mRNA expression.Results The impacts on TCF-β1mRNA: the proportion of TCF-β1mRNA positve cells on the 7th day AM and the 28th day IM show that TCM therapy can evidently inhibit the alveolar macrophage and lung cell of Leydig to expressed through TCF-β1mRNA，therefore， the airsacculitis and fibrotic process are inhibited（P＜0.05).Conclusion The mechanism of QFHT Principle，YQHX Principle， two method associated usages and two method to induct by stages on preventing pulmonary fibrosis is to inhibit the process of airsacculitis and pulmonary fibrosis. And the performance of the mechanism is showed by decreased the expression of TGF-β1mRNA in alveolar macrophage and alveolar interstitial macrophage.