蛋内注射CDCA对鸡胚胎期骨骼肌发育的影响

he meat production potential of animal is closely related to muscle fiber number. The number of muscle fibers in avian and mammal skeletal muscle was determined by the degree of embryonic myoblast proliferation and differentiation, therefore the research on skeletal muscle development in avian embryos not only has important academic value，but also has great significance in improving the meat production potential.In order to explore the effect of in ovo infection of CDCA on avian skeletal muscle development, 240 White Plymouth Rocks breeder eggs on day 3 of embryonic age were selected and allocated to four groups randomly, which were injected in albumen with 500ng/50μL CDCA (low- dose group), 1000ng/50μL CDCA (high-dose group), DMSO (negative group) or 50μLPBS (control group). The results showed in ovo injection with 500 ng of CDCA significantly increased body weight, liver weight, leg muscle weight of broiler on day 42 and leg muscle fiber density on E16 chick embryo and DOH (day of hatch). There was an increase in chick weight on DOH and leg muscle fiber diameter of broiler on day 42 in the group of injected with 1000ng/50μL CDCA. Results of RT-PCR showed that the mRNA expression levels of decorin and myogenic regulatory genes (Myf-5, MyoD and Myogenin) were significantly upregulated in leg muscle of low-dose group on DOH and D42. These results suggested in ovo injection of CDCA could promote development of muscle fiber and improve meat production in broilers.