大骨节病死亡受体途径调节因子表达的组织学改变

Objective To observe the expressive changes of Fas-associated death domain protein and Cellular-FLICE inhibitory protein in articular cartilage with Kashin-Beck disease (KBD) and it's role in the pathogenesis of the disease. Methods The cartilage samples collected from patients based on the diagnosis criteria of KBD and osteoarthritis, were divided into three groups, including KBD, osteoarthritis and the healthy control, respectively. The expressive changes of Fas-associated death domain protein (FADD) and Cellular-FLICE inhibitory protein (c-FLIP) in cartilage were stained by immunohistochemistry, and the positive chondrocytes counted in the different zones in articular cartilage among three groups under microscope. Results 1.The positive expressive rates of FADD in the middle zone of articular cartilage from patients with KBD（28.68±2.19） and osteoarthritis （35.40±2.34）were high than significantly that in normal cartilage（10.51±5.02, F=16.245, P=0.000), but no significance in upper and deeper zones among the three groups (P= 0.206~0.761). 2.The FADD expression was highest in middle zone (28.68%±5.38%) , and followed by deeper zone(17.94%±8.38%) in KBD cartilage 3. The FLIP expression in upper zone of cartilage from KBD and OA was high than significantly that in the healthy control (F=5.929, P=0.018), and not different in middle and deep zones comparing to the healthy cartilage. Conclusion There is significant increased in the FADD expression of middle zone ，but decreased in FLIP expression in upper zone in KBD cartilage, and shows that death receptor pathway and it's regulator have an important role in the pathogenesis of KBD.