前列腺素PGE2对肝癌细胞CD29表达调控及相关机制的研究

Objective: To investigate the effect of prostaglandin E2 (PGE2) on the expression of CD29 and its related signaling pathway by EP1 receptor in Huh-7 cells. Methods: Huh-7 cells were treated with PGE2, EP1 receptor agonist（17-phenyltrinor Prostaglandin E2）, EP1 receptor antagonist SC19220,NF-κB inhibitor PDTC,Western blot and immunofluorescence test were employed to detect the expression of CD29 and activation of NF-κB in Huh-7 cells.Results: The level of CD29 was increased by 129.48%(P<0.01) after being treated with 5μmol/L PGE2 for 24h, The level of CD29 was increased by 216.34%（P<0.01）after being treated with EP1 receptor agonist (5μmol/L ) for 24h. the expression level of CD29 was decreased by 79.19% (P<0.05) after being treated with EP1 receptor antagonist SC19220 (10μmol/L). The expression lever of cell nucleus was obviously increased for 120min by immunofluorescence; The level of Phospho-NF-κB-P65 was increased through Western blot. After the treatment of NF-κB inhibitor PDTC , the level of CD29 was decreased by 94.95% (P<0.01). Conclusion: PGE2 might up-regulate the expression level of CD29 through EP1 receptor in Huh-7 cells, which could be partly related to the NF-κB signaling pathway.