A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes
A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes
Abstract Enterovirus G (EV-G) belongs to the family of Picornaviridae. Two types of recombinant porcine EV-Gs carrying papain-like cysteine protease (PLCP) gene of porcine torovirus, a virus in Coronaviridae, are reported. Type 1 recombinant EV-Gs are detected in pig feces in Japan, USA, and Belgium and carry the PLPC gene at the junction site of 2C/3A genes, while PLPC gene replaces the viral structural genes in type 2 recombinant EV-G detected in pig feces in a Chinese farm. We identified a novel type 2 recombinant EV-G carrying the PLCP gene with flanking sequences in place of the viral structural genes in pig feces in Japan. The ~0.3 kb-long upstream flanking sequence had no sequence homology with any proteins deposited in GenBank, while the downstream ~0.9 kb-long flanking sequence included a domain having high amino acid sequence homology with a baculoviral inhibitor of apoptosis repeat superfamily. The pig feces, where the novel type 2 recombinant EV-G was detected, also carried type 1 recombinant EV-G. Although the phylogenetic analysis suggested that these two recombinant EV-Gs have independently evolved, type 1 recombinant EV-G might have served as a helper virus by providing viral structural proteins for dissemination of the type 2 recombinant EV-G.
Imai Ryo、Mizutani Tetsuya、Masuda Tsuneyuki、Oba Mami、Tsuchiaka Shinobu、Katayama Yukie、Makino Shinji、Nagai Makoto、Yamazato Hiroshi、Sakaguchi Shoichi、Naoi Yuki、Kuroda Moegi、Omatsu Tsutomu
Research and Education Center for Prevention of Global Infectious Disease of AnimalResearch and Education Center for Prevention of Global Infectious Disease of AnimalKurayoshi Livestock Hygiene Service CenterResearch and Education Center for Prevention of Global Infectious Disease of AnimalResearch and Education Center for Prevention of Global Infectious Disease of AnimalResearch and Education Center for Prevention of Global Infectious Disease of AnimalDepartment of Microbiology and ImmunologyAzabu UniversityKurayoshi Livestock Hygiene Service CenterResearch and Education Center for Prevention of Global Infectious Disease of Animal||Osaka Medical CollegeResearch and Education Center for Prevention of Global Infectious Disease of AnimalKurayoshi Livestock Hygiene Service CenterResearch and Education Center for Prevention of Global Infectious Disease of Animal
微生物学分子生物学生物科学研究方法、生物科学研究技术
enterovirustorovirusrecombination
Imai Ryo,Mizutani Tetsuya,Masuda Tsuneyuki,Oba Mami,Tsuchiaka Shinobu,Katayama Yukie,Makino Shinji,Nagai Makoto,Yamazato Hiroshi,Sakaguchi Shoichi,Naoi Yuki,Kuroda Moegi,Omatsu Tsutomu.A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/510131.点此复制
评论