隐丹参酮对宫颈癌Hela细胞的放射增敏作用

Objective To investigate the radiosensitization effects, the cell cycle and apoptosis induction of cryptotanshinone on Hela cell line of cervical cancer. Methods The MTT assay was used to detect the growth inhibition rates of Hela cells at different doses of 0,1,2,4,8Gy which cultured with cryptotanshinone (4.03µg/ml). With multi-target single-hit model, The radi-osensitization effect was investigated by clone formation assay through sensitizing enhancement ratio(SER). The cell cycle distribution and apoptosis were tested by flow cytometry. Results At different irradiation doses,the cryptotanshinone had cytotoxicity on Hela cells, which were clearly dose-dependent. The IC20 of cryptotanshinone(4.03µg/ml) indicated the radiosensitization on Hela cell line. Further clone formation assay indicated that the sensitization enhancement ratio was 1.4.Cell treated with cryptotanshinone, which indicated significant Go/G 1 phase arrest and a decrease in S phase. Conclusions Cryptotanshinone had significant cytotoic and radio- sensitization effects on Hela cell. One of the mechanism maybe that it might have significant effects on cell cycle and induce apoptosis of Hela cell, have significant on G0/G1 phase arrest and a decrease in S phase which cells were more sensitive to radiation.