烟草半胱氨酸蛋白酶基因家族和相应 miRNAs 的鉴定及其对 PVY 的响应

Potato virus Y (PVY) is a representative species of Potyviridae and Potyvirus, and is one of the major plant viruses that seriously harm the yield and quality of tobacco and other solanIdentification of the cysteine protease family and correspondingaceous crops in production. In this study, the cysteine protease gene family of Nicotiana tabacum L. and their corresponding miRNAs were identified at the genome-wide level. And then analyze their expression characteristics after PVY infection. The results showed that a total of 70 cysteine protease genes were identified in Nicotiana tabacum L. genome, which could be divided into five subfamilies (C1A, C2, C12, C13 and C14).Based on the sequencing results of small RNA-seq, 51 miRNAs were found to be targeted to 28 members of cysteine protease gene family, 7 of the cysteine protease genes were negatively correlated with miRNAs after PVY infection. The results of qRT-PCR showed that 15 cysteine protease genes were significantly up-regulated after PVY infection. This study will be helpful for better understanding the function of cysteine protease gene family in Nicotiana tabacum L. and the interaction of the family genes with their corresponding miRNAs, and how this interaction regulates the response of Nicotiana tabacum L. to PVY.