长链非编码RNA PTENP1 在膀胱癌发生发展中的分子机制

Objective To explore the molecular mechanism underlying the biological function of lncRNA PTENP1 in bladder cancer. Methods Expressions of PTENP1, PTEN and miR-17 were examined by quantitative reverse transcriptase PCR (qRTPCR) in 12 bladder cancer tissues. The expression of PTEN was examined by Western blotting in bladder cancer cell lines T24 and 5637 overexpressing PTENP1. Luciferase reporter assay was performed to confirm the targeting of miR-17 to PTENP1 and PTEN. T24 and 5637 cell lines with stable overexpression of PTENP1 and mir-17 were used to investigate effect of PTNE and miR-17 on the function of PTENP1 in bladder cancer. Results The expression of miR-17 was up-regulated and PTENP1 and PTEN were down-regulated in bladder cancer tissues, where a positive correlation was found between PTENP1 and PTEN expressions and a negative correlation between PTENP1 and miR-17 (P<0.05). Overexpression of PTENP1 in bladder cancer cell lines T24 and 5637 obviously enhanced the expression of PTEN protein. miR-17 was found to target both PTENP1 and PTEN and promote the growth of bladder cancer. miR-17 could partially restore the tumor-suppressing activity of PTENP1 in bladder cancer. Conclusion By binding with miR-17, lncRNA PTENP1 functions as a PTEN competing endogenous RNA (ceRNA)tosuppresstheprogressionofbladdercancer.