超表达大豆植酸酶Sphy1基因对转基因烟草植株利用有机态磷能力的影响

ue to its huge amount in the soil, the phytate is an important potential source for providing the plants with available phosphorus (Pi) by the catalytic action involved of phytase. In this study, the construct fused the open reading frame (ORF) of Sphy1 into corresponding positions in frame of binary expression vector pBI121 was created and used to transform tobacco. Molecular identification by PCR and RT-PCR indicated the target gene Sphy1 in transgenic tobacco plants to be transcribed under the regulation of upstream promoter. Compared to the control plants, the phytase activities in all the transgenic plants were increased, with the increase range in consistently with the expression levels in the transgenic plants. Under the growth condition in which the phytate to be the sole phosphorus source, the line 1 plants which displayed high expression level of Sphy1 showed a noted better growth performance, such as higher fresh weight and dry weight, as well as higher total P content and more accumulative P amount per plant than CK. This clearly indicated that overexpression of Sphy1 could improve the phosphorus acquisition by the extruded Sphy1 phytase in the rhizosphere, where this enzyme catalyze the degradation of the phytate and release the available Pi for plants. The Sphy1 gene is seemingly to have potential value in the creation of new crop cultivars with high phosphorus use efficiency.