亮氨酸对卫星细胞mTOR信号通路的影响
Effect of leucine on mTOR signaling in myogenic satellite cells
本研究探讨亮氨酸对卫星细胞mTOR信号通路关键激酶的影响。在提取的肌源卫星细胞中加入亮氨酸，采用免疫印迹的方法，考察了2m mol/L亮氨酸对卫星细胞mTOR、S6K、4EBP1、TSC2和Akt的蛋白磷酸化水平同时检测了对蛋白质合成和DNA合成速率的影响。结果可知，肌源卫星细胞在加入亮氨酸作用下，mTOR、S6K、4EBP1、TSC2和Akt的磷酸化明显激活，而采用mTOR的特异性抑制剂雷帕霉素处理细胞，能够立刻中止蛋白和DNA的合成，此时亮氨酸的激活无效。也就是说，亮氨酸可以通过激活mTOR信号通路关键激酶，从而活化卫星细胞。
he effect of leucine on crucial kinases of mTOR signal pathway was investigated. 2m mol/L Leucine was added in satellite cells from pig, and western blotting was chosen to illuminate the influences of leucine on phospharylation of mTOR、S6K、4EBP1、TSC2 and Akt, at the same time, protein synthesis and DNA proliferation were conducted. Results shown, phospharylation of mTOR、S6K、4EBP1、TSC2 and Akt were significantly increased, Rapamycin as the inhibitor of mTOR was added in satellite cells, protein synthesis and DNA proliferation were all terminated in spite of activation of leucine. In a word, leucine can activate satellite cell by activating crucial kinases of mTOR signal pathway.
韩冰、叶暾昊、石彦国、张根生、韩春然、李次力
细胞生物学生物化学分子生物学
亮氨酸卫星细胞mTOR信号通路
leucinesatellite cellmTORsignal pathway
韩冰,叶暾昊,石彦国,张根生,韩春然,李次力.亮氨酸对卫星细胞mTOR信号通路的影响[EB/OL].(2013-07-12)[2025-07-21].http://www.paper.edu.cn/releasepaper/content/201307-171.点此复制
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