金黄色葡萄球菌α-毒素对肺水通道AQP1表达的影响
he effect of S.aureus α-toxin on pulmonary aquaporin-1 expression in mice.
目的 观察金黄色葡萄球菌α-毒素（α-Toxin）对Balb/c小鼠肺微血管内皮细胞（LMEC）水通道蛋白-1（AQP1）表达的影响。方法 将实验组小鼠15只腹腔注入α-Toxin后根据6h、10h、24h取材时间随机将它们分为α-Toxin 6h组、α-Toxin 10h组和α-Toxin 24h组。取左肺行病理HE染色和免疫组化染色，检测肺组织病理变化情况和AQP1表达的变化。结果 α-Toxin各组肺泡间隔增宽、水肿，炎细胞浸润，肺泡结构破坏，以α-Toxin 10h组最为明显。免疫组化α-Toxin各组AQP1表达下降（p<0.05）。其中以α-Toxin 10h组最为明显。α-Toxin 6h组和α-Toxin 10h组及α-Toxin 10h组和α-Toxin 24h组均有显著性差异（p<0.05）。结论 AQP1表达于LMEC。金葡菌α-毒素可致小鼠肺LMEC的AQP1表达下降，随着炎症的减轻，AQP1也随之表达增加，进而恢复正常。
Objective: To observe the effect of S.aureus alpha toxin on pulmonary aquaporin-1 (AQP1) expression in mice. Methods: Balb/c mice weighted 18~27g was divided into alpha toxin-6h group, alpha toxin-10h group and alpha toxin-24h group according to the time point after injecting alpha toxin peritoneally. Immunohistochemistry stain for pulmonary AQP1 was done after execution. Results: Alveolar wall showed edema, inflammatory cell infiltration in all alpha toxin groups, especially in alpha toxin-10h group. AQP1 expression decreased in these groups, in which alpha toxin-10h group was more obvious. Conclusion: AQP1 is mainly localized on lung microvascular endothelial cell. AQP1 expression decreased after mice exposed to S.aureus alpha toxin, while down-regulated AQP1 returned to normal level overtime.
夏淑杰、才丽萍、李玲、林庶茹、李艳杰、王秋月、李尔然、康健
基础医学微生物学
α-毒素（α-Toxin）水通道蛋白1（AQP1）肺微血管内皮细胞（LMEC）
α-Toxin Aquaporin Lung mirovaseular endothelial cell
夏淑杰,才丽萍,李玲,林庶茹,李艳杰,王秋月,李尔然,康健.金黄色葡萄球菌α-毒素对肺水通道AQP1表达的影响[EB/OL].(2007-04-28)[2025-08-06].http://www.paper.edu.cn/releasepaper/content/200704-720.点此复制
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