粒细胞-巨噬细胞集落刺激因子（GM-CSF）微球制剂的体外释放研究

In this paper, a polysaccharose aqueous phase-aqueous phase emulsion was used to encapsulate GM-CSF into polysaccharide particles which are able to withstand the harsh conditions. And then these glassy particles were microencapsulated into PLGA microspheres using solid-in-oil-in-hydrophilic oil(S/O/hO). The results showed that size of the microspheres was about 10 μm in diameter and had a narrow size distribution.Meanwhile,the release test in vitro provided evidence that the microspheres, with an encapsulation efficiency of 83.79 % and low burst release, achieved a sustained release of about one month.