拟南芥CML24参与NO调控花粉管转向的实验证据

Nitric oxide (NO) is a gasous signal molecule in plant cells, and it plays essential roles in plant growth and development as well as in pollen tube growth and guidance for double fertilization. CML24 was found positively to be involved in pollen grain germination and pollen tube tube elongation. In the current report, we found that, in comparision to wild type, the cml24-4, the loss-of-function mutant of CML24, showed much higer rate of abnormal pollen tubes, less probability of the tubes to target female gametophytes and much lower seed setting rate. Further data showed that the cml24-4 mutant pollen tubes accumulated much higher concentration of NO and displayed hyposensitivity to NO induced pollen guidance. Our data suggest that the CML24 is a positive component of NO regulated pollen tube growth and orientation for successful double fertilization and seed setting.