猪SOCS1基因多态性分析

In this study, SOCS1 gene was selected as a candidate gene for porcine anti-disease traits. Direct sequence method was used to detect the polymorphism of SOCS1 gene. The analysis of gene frequency and polymorphic information content were performed in Minzhu and Landrace pig populations. A C/T mutation in the 5'UTR region of this gene was found and a DpnI PCR-RFLP assay was established to detect this mutation. Statistics analysis showed that there were three genotypes in Minzhu and Landrace populations and the C allele frequency was 0.42 and 0.51 respectively. The polymorphic information content was moderate diversity in both Minzhu and Landrace. Association analysis between this mutation and anti-disease traits in different pig populations will be performed in future research.