姜黄素对过氧化氢诱导小鼠脑神经瘤细胞氧化损伤的保护作用

he neuroprotective effects and its potential mechanisms of curcumin against H2O2-induced oxidative damage in mouse neuroblastoma Neuro-2A cells was studied. Neuro-2A cells were seeded into culture plates, and pretreated with curcumin at different concentrations prior to 30 μmol/L H2O2 exposure for 24 h. Cell viability was measured by MTT assay, cell apoptosis was assessed by nuclear staining, the level of intracellular total antioxidant capacity (TAC) was determined by colorimetric method, and mitochondrial membrane potential was tested by flow cytometry. Results showed that pretreatment with 25 μg/mL curcumin for 1 h increased the percentage of viable cells by 8.5%, reduced the percentage of apoptotic cells by 15.1%, elevated TAC by 15.9%, and decreased the percentage of the Rhodamine 123 negative cells by 11.81%. It is concluded that curcumin is endowed with significant protective effects against H2O2-induced oxidative stress and apoptosis in Neuro-2A cells by its ability to enhance TAC, and inhibit the loss of mitochondrial membrane potential, and might be a therapeutic agent for prevention and treatment of neurodegenerative diseases implicated with oxidative stress.