一种基于萘酰亚胺的用于活细胞内微粘度成像的荧光探针
naphthalimide based fluorescent probe for imaging microviscosity in living cells
细胞内粘度严重影响着细胞内物质的运输和信号的传导，并对生物大分子之间的反应和活性代谢物的扩散产生重要影响。因此，我们设计合成了一种基于萘酰亚胺-半花箐染料的荧光探针（Cyt-NA）并用于活细胞内粘度变化的成像。该探针对粘度由1.0 cP增加到1410 Cp 时具有良好的荧光信号增强变化，并将其用于监控在温度改变和细胞饥饿状态下细胞内微粘度的变化，从而为研究细胞内微粘度的变化提供了一种有效的工具。
Intracellular viscosity strongly influences transportation of mass and signal, interactions between the biomacromolecules and diffusion of reactive metabolites in live cells. Herein, we developed a naphthalimide-hemicyanine based fluorescent probe Cyt-NA for imaging the change of microviscosity in living cells. Cyt-NA exhibited about 260 folds fluorescence increasing at 610 nm along with the increasing viscosity from 1.0 cP to 1410 cP. These satisfying response properties make Cyt-NA possible to monitor viscosity changes under starvation and low temperature conditions, offering us more practical information about microenviromental viscosity in living cells.
张小玲、郭秉鹏
生物物理学生物科学现状、生物科学发展生物科学研究方法、生物科学研究技术
荧光探针微粘度细胞成像
fluorescent probemicroviscositycell imaging
张小玲,郭秉鹏.一种基于萘酰亚胺的用于活细胞内微粘度成像的荧光探针[EB/OL].(2017-04-28)[2025-06-06].http://www.paper.edu.cn/releasepaper/content/201704-794.点此复制
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