一个新的家蚕LIM蛋白基因的序列分析和原核表达
Sequence analysis and prokaryotic expression of a novel LIM protein gene from silkworm, Bombyx mori
LIM蛋白是一类含有LIM结构域的真核蛋白家族，它可介导细胞分化和发育，转录调控和激活、参与细胞骨架形成等多种重要的生物调节过程。从家蚕蛹cDNA文库获得一个新的编码LIM only蛋白的基因，其GenBank登录号为DN6907984，我们将其命名为BmLIMO（LIM only protein）。该基因全长为627bp，编码长度为186个氨基酸残基的蛋白质，预测分子量为21.6kDa，等电点为9.139。我们对BmLIMO基因进行了生物信息学方面的分析，并用DNA重组技术将BmLIMO片段克隆到原核表达载体pET-28a中，在大肠杆菌BL21(DE3)中成功表达了可溶性BmLIMO蛋白，这为我们进一步研究该蛋白功能奠定了基础。
he eukaryotic LIM protein family is characterized by one or more conserved LIM domains that has been shown to be critical for the cellular differentiation and development, transcriptional control and the cytoskeleton formation. We identified a gene encoding an LIM protein from a cDNA library of silkworm pupae, whose GenBank Accession Number is DN6907984, and we named it BmLIMO(LIM only protein). The full length of the gene is 627bp, and it encodes 186 amino acids, with their predicted mass 21.6kDa and isoelectric point 9.139. After bioinformatic analyses on BmLIMO, we cloned the fragments of interest into the expression vector pET-28a and transformed into E.coli BL21(DE3) to induce protein expression. This will provide us important evidence to make further studies of BmLIMO.
陈剑清、沈亚芳、黄宏杰、何靖、张耀洲
分子生物学生物工程学昆虫学
家蚕LIM only生物信息学分析原核表达
Bombyx moriLIM onlyBioinformatic analysesProkaryotic expression
陈剑清,沈亚芳,黄宏杰,何靖,张耀洲.一个新的家蚕LIM蛋白基因的序列分析和原核表达[EB/OL].(2009-03-12)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/200903-430.点此复制
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