新生大鼠缺氧缺血性脑损伤TLR4表达及与细胞凋亡的关系

Objective To investigate the role of TLR4 in HIBD by observing the change of TLR4 expression and cell apoptosis after hypoxic-ischemic brain damage (HIBD)in a neonatal rat model, and provide an experimental foundation for the futher clinical research. Methods A total of 80 7day postnatal Sprague-Dawley rats were randomly divided into experimental group(n=40) and control group(n=40). The expression of TLR4 was tested by immunohistochemistty the apoptotic hippocampus cells were tested by TUNEL. Results The cell apoptosis and the expression of TLR4 increased at HI 6 h, and achieved the hightest increases at HI 12 h, and it continued to maintain the high level in HI 24 h, then decreased at HI 72 h,HI7 d group. There were significant differences of positive rate at different times compared with control group(P<0.05). There was a positive correlation between apoptosis and TLR4(r=0.452) in rats with hypoxic-ischemic brain damage. Conclusion TLR4 over expressed in HIBD can promote cell apoptosis, and play an important role in the pathogenesis of HIBD.