血红素加氧酶-1介导Ca2+信号调控的黄瓜不定根发生

In this report, the role of heme oxygenase-1 (HO-1) in Ca2+ signal-induced cucumber adventitious root was investigated by pharmacological, physiological and molecular techniques. Our results showed that both of hemin (an inducer of HO-1) and calcium chloride (CaCl2) could induce cucumber adventitious root formation in a dose-dependent manner. Meanwhile, the up-regulation of cucumber HO-1 gene transcripts was observed. However, ZnPP, an inhibitor of HO-1, was able to inhibit cucumber adventitious root formation and down-regulate cucumber HO-1 gene expression. In a subsequent experiment, we discovered that Ca2+ chelator (EGTA) and Ca2+ channel inhibitors (LaCl3 and RR) treatment not only blocked cucumber adventitious root formation, but also decreased HO-1 protein level. In conclusion, the results presented in this paper suggested the involvement of cucumber HO-1 in the Ca2+-induced adventitious rooting.