水稻籽粒灌浆过程中蛋白质表达特性及其对氮肥运筹的响应

he 2-D PAGE was employed to separate proteins of grain in the different grain-filling stages of large panicle rice. Then, the different nitrogen application ratios (7:3 and 6:4) were set to clarify the different response mechanisms of superior and inferior grains. 2D maps of grain proteome at different time courses during grain-filling stage were got with 32 differentially expressed proteins were detected by Imagemaster software, while 27 proteins were successfully identified by MS. Then, differential expression patterns proteins in superior and inferior grains at different time courses responding to different N application ratios were further analysis. The results showed 26 differentially expressed proteins with 17 identified in inferior grains, while 9 proteins with differential expressions were detected in superior grains and 4 were successfully identified. Grain-filling is a complicated ecological process, involving starch synthesis, energy metabolism, signal transduction, genes expression and regulation and so on. These molecular mechanisms act together to advance the seed whole development, from germination to vegetative development, productive development, and to the natural senescence. The grain-filling of superior spikelet showed environmental stability, but the grain-filling of inferior spikelet could be regulated by the environment factor (such as Nitrogen application).The increasing N application amounts in grain-filling phases of rice favored in the up-regulation of the genes involved in physiological activities of inferior grain filling, including the signals transduction, gene expression, energy accumulation for the material metabolism, and hormone production etc. in inferior grains of rice, in turn, leading to increased yield.