richostatin A对鸡胚翅芽发生中基因表达模式的作用

Epigenetic controlling and chromatin remodelling are involved in embryonic genesis and tumorigenesis. Acetylation is one of the key modifications to control gene transcription. HDACs inhibitor is considered to be among the most promising targets in drug development for cancer therapy. However, that basic aspects of what kinds of genes are more sensitive to the modification of acetylation in vivo are not fully understood. Taking chicken embryonic limb as an experimental model, the reaction of a batch of genes has been investigated in the limbs treated with Trichostatin A (TSA), a histone deacetylase (HDAC)-inhibitor. The results show that TSA (75 µM) changes the expression levels of the genes, which have important functions during limb development. Among them, SF/HGF and Twist1 were up-regulated, and FGF8, Shh, TCF4, Myf5 and MyoD were down-regulated. In contrast to that, the Pax3, Paraxis, CREB, and PCNA were still expressed at the same levels. Chicken limb development can be served as a convenient model in vivo for studying the effect of HDAC inhibitors on gene expression. It may be useful for improving our understanding of the role of chromatin remodelling and epigenetic control of gene expression patterns and developing the drugs against cancer.