拟南芥PSRP2调控干旱胁迫研究
rabidopsis thaliana PSRP2 regulates drought stress
植物逆境胁迫不仅影响植物的生长发育,同时会对农作物产量造成极大的威胁。在实验室前期研究中发现FOF2能够参与植物的干旱胁迫,但是FOF2调控干旱胁迫的机制仍不清楚。本研究通过IP质谱筛选到了FOF2的互作蛋白PSRP2。干旱处理后PSRP2的表达量降低。psrp2突变体具有耐旱表型,而PSRP2的过表达株系则对干旱胁迫敏感,表明PSRP2在植物干旱胁迫响应中起负调控作用。转录组测序和实时荧光定量PCR分析结果显示PSRP2与FOF2共同调控下游非生物胁迫相关基因的表达。这些研究结果初步表明PSRP2与FOF2在同一条通路上调控干旱胁迫响应。
Plant stress not only affects the growth and development of plants, but also poses a great threat to crop yield. In previous laboratory studies, it was found that FOF2 can participate in drought stress in plants, but the mechanism of FOF2 regulation of drought stress is still unclear. In this study, the interaction protein PSRP2 of FOF2 was screened by IP mass spectrometry. The expression of PSRP2 decreased after drought treatment. The psrp2 mutant had a drought-tolerant phenotype, while the overexpressing lines of PSRP2 were sensitive to drought stress, indicating that PSRP2 played a negative regulatory role in the response of plantsArabidopsis thaliana PSRP2 regulates drought stress to drought stress. The results of transcriptome sequencing and real-time PCR analysis showed that PSRP2 and FOF2 jointly regulated the expression of downstream abiotic stress-related genes. These results preliminarily suggest that PSRP2 and FOF regulate drought stress response in the same pathway.
李新梅、赵小英、苗卓文、段菲菲、屈丽娜、周泉谕
植物学农业科学研究生物科学研究方法、生物科学研究技术
分子生物学干旱胁迫FOF2PSRP2
molecular biologydrought toleranceFOF2PSRP2
李新梅,赵小英,苗卓文,段菲菲,屈丽娜,周泉谕.拟南芥PSRP2调控干旱胁迫研究[EB/OL].(2024-04-01)[2025-08-19].http://www.paper.edu.cn/releasepaper/content/202404-27.点此复制
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