辣椒NADPH 基因在辣椒中的遗传转化及其抗病性鉴定
NADPH Gene Transformated to Pepper and Transgenic Plants Identification of Resistance to Phytophthora capsici
以辣椒NADPH基因为目的基因，利用载体pBI121构建了植物表达载体pBI121-NADPH，采用农杆菌介导法将其转化辣椒(Capsicum annuum L. )感病品种B12，对卡那霉素抗性转化植株进行PCR和RT-PCR分子检测，获得了5个转化NADPH基因的转基因系。应用辣椒疫病抗性离体叶片鉴定技术，对转基因植株的抗病性进行了鉴定。结果表明，转基因辣椒的发病率较非转基因植株降低了22.2%，其病情指数降低了46.5%。
he pBI121-NADPH plant gene expression vector was constructed by using the vector pBI121.Pepper susceptible Cv.B12 was transformed with pBI121-NADPH by Agrobacterium tumefaciens-mediated transformation. Kanamycin-resistant regenerated lines were obtained, PCR and RT-PCR molecule detection confirmed that the five transgenic lines carried the target gene. The resistance of transgenic plants to Phytophthora capsici was identified with in vitro leaf technique. Results showed that the incidence of transgenic plants were reduced about 22.2% than those of the non-transgenic peppers, the disease index of transgenic plants were also reduced 46.5%
包良帅、李大伟、巩振辉、柴贵贤
植物保护遗传学生物工程学
辣椒NADPH基因遗传转化疫霉菌抗病性鉴定
pepperNADPH genegenetic transformationPhytophthora capsiciResistance Identification
包良帅,李大伟,巩振辉,柴贵贤.辣椒NADPH 基因在辣椒中的遗传转化及其抗病性鉴定[EB/OL].(2009-12-30)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200912-1169.点此复制
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