P16在高氧致CLD早产鼠肺组织表达及意义
Expression and significance of p16 in premature rats w ith Chronic Lung Disease Induced by Hyperoxia
目的:探讨p16基因及蛋白在高氧致早产鼠慢性肺疾病(CLD)中表达及意义。方法:建立高氧诱导早产鼠CLD模型, 80只早产鼠被随机分为实验组及对照组,分别应用免疫组化及RT-PCR技术测定肺组织p16 mRNA及蛋白表达。结果: 暴露高氧后的7天，肺组织的p16的基因及蛋白的表达水平开始低于对照组，并且随着高氧暴露时间的延长，p16的基因及蛋白表达水平逐渐减少，于暴露后的21天达最低值。结论: 暴露高氧中早产鼠，其肺组织的p16 mRNA及蛋白的表达水平降低，其低水平的表达状态与高氧致CLD肺纤维化的发生密切相关。
Objective:To explore the expression and significance of p16 in premature rats with chronic lung disease(CLD) induced by hyperoxia. Methods: 80 premature rats were randomly divided into experimental group with CLD induced by hyperoxia, and control group(n=40). The expressions of p16 protein and mRNA were detected by using immunohistochemical methods and RT-PCR. Results:The expressions of p16 protein and mRNA was lower in the experimental group than those in control group at day 7 , and then gradually decreased with extended exposure to hyperoxia ,but reached the lowest levels at day 21 after hyperoxia. Conclusion: The p16 protein and mRNA expressions were downregulated in premature rats exposure to oxygen. Its downregulation was closely correlated with the development of lung fibrosis.
薛辛东、岳晓红、富健华
基础医学
p16慢性肺疾病早产
p16chronic lung diseasepremature
薛辛东,岳晓红,富健华.P16在高氧致CLD早产鼠肺组织表达及意义[EB/OL].(2010-01-05)[2025-06-07].http://www.paper.edu.cn/releasepaper/content/201001-128.点此复制
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