丹酚酸B对MC3T3-E1细胞Cbfa1基因表达的影响及Cbfa1启动子活性的调节

Objective Explore the effects of salvianolic acid B(SalB) on the mRNA expression of Cbfa1 gene in MC3T3-E1 cell line and the activity of Cbfa1 promoter. Methods The mouse MC3T3-E1 cells were treated for 24hours or 48hours with SalB of different concentration respectively. The mRNA expression of Cbfa1 gene were examined by using real-time RT-PCR. Luciferase reporter vector of mice Cbfa1 promoter was constructed and then transfected into MC3T3-E1 cells. The action of SalB on the activity of Cbfa1 promoter were evaluated by monitoring the expression of luciferase. Results We demonstrated in MC3T3-E1cells, 0.1μM and 10μM SalB increased Cbfa1 mRNA expression after treatment for 24hours and 48hours（F=23.24，P<0.01）. The maximum increase of Cbfa1mRNA level is 1.71-fold greater than that of contol under intervention of 0.1μM SalB for 48hours（t=10.36，P＜0.01）. Compared with the control, the promoter activity of Cbfa1 gene were upregulated to 2.63-fold and 1.89-fold respectively after treating with 0.1μM and 10μM SalB（F=13.27，t= 5.83、4.53，P<0.01）. Conclusions SalB can increase Cbfa1 gene expression and promote Cbfa1 promoter activity in MC3T3-E1 cells.