黄芪对大鼠胫前肌失神经肌萎缩的防治研究

Objective The preventive and therapeutic effects of Astragalus on the denervated tibial muscle atrophy of rats were studied. Methods After 60 Sprague-Dawley rats were subjected to Common Peroneal nerve crush model of 5 mm injury, and randomly divided into 6 groups for daily intragastric administration of drugs：Astragalus high-dose, medium-dose, low-dose groups, mecobalamin group (positive control), model group, sham operation group. The drug administration lasted for 18 days. 18 days after operation, slice, staining and analysis morphology were performed. The wet weight ratio and section area of tibial muscle werelso measured. At the same time, qRT-PCR detect the differential expression of Angptl4 and PI3K in the high-dose group of Astragalus and model group. Results ①Wet weight ratio：The wet weight ratio in Astragalus group and in mecobalamin group all increased significantly compared with that of model group（P＜0.01）．②Tibial muscle section area: Sham operation group was large, morphous was regular; while model group significantly diminished, structure was chaotic, and connective tissue hyperplasia was more obvious; Astragalus group and mecobalamin group fairly diminished, morphous was fairly regular, and connective tissue hyperplasia was not obvious. Compared with model group, Astragalus group all increased significantly (P < 0.01). ③The motor end-plate: The sham operation group were sepia、oval or round; the Astragalus high-dose group and mecobalamin group has little chage; the Astragalus medium-dose group、low-dose group and the model group had lessen, shape was not rule gradually, the edge were rough, coloring were slight gradually. ④The expression of Angptl4 and PI3K: Compared with the model group, the Astragalus high-dose increased significantly(P<0.05). Conclusion Astragalus has a significant effect of prevention and therapy on denervated tibial muscle atrophy of rats. Astragalus maybe increase Angptl4 and PI3K expression to delay the denervated tibial muscle atrophy of rats.