G蛋白偶联受体17对脑缺血损伤大鼠iNOs、TNF-α表达的影响

Objective To observe the effect of G protein coupled receptor 17 (GPR17) on the expression of inducible nitric oxide synthase (iNOs) and tumor necrosis factor-α (TNF-α) during cerebral ischemic injury. Method Male Sprague-Dawley rats were randomly divided into 4 groups: control group, saline group, NC group and GPR17 siRNA group. Because no GPR17 selective antagonist is currently available, RNA interference strategy was used in vivo to evaluate the regulatory role of GPR17. Ischemic brain injury was induced by middle cerebral arterial occlusion (MCAO) methods. The expression of iNOs and TNF-α was detected by immunohistochemistry, respectively. Results Compared with the control group, the expression of iNOs and TNF-α in the ischemic area were significantly elevated (P <0.01). Compared with the saline group, GPR17 intervention significantly reduced the cerebral ischemia injury, and inhibited the increased expression of iNOs and TNF-α in ischemic area (P <0.01). Conclusion GPR17 siRNA attenuates cerebral ischemic injury and inhibits the expression of inflammatory mediators in ischemic area.