纳米Y/HA修饰纯钛对HGF生物活性及其抗菌研究

he repair and induced regeneration of peri-implant soft tissues play important roles in long-term and short-term clinical successes of implants. Surface modification with coatings is an effective approach to improving the biocompatibility and antibacterial properties of implants.In this study, we introduced a novel surface modification method for pure titanium (Ti)-based implant materials. We also examined and assessed the biocompatibility of the resulting modified materials with human gingival fibroblasts (HGFs), as well as their antibacterial properties against Streptococcus mutans. Hydroxyapatite (HA) and HA doped with different concentrations of yttrium (Y) were coated on the surface of pure Ti substrate via sol-gel process. The surface of those samples was characterized and analyzed via scanning electron microscope (SEM), atomic force microscope (AFM), contact angle meter, X-ray photoelectron spectroscopy (XPS), and X-ray diffractometer (XRD). The effects of pure Ti substrate coated with HA containing different concentrations of Y on the adhesion, proliferation, morphology, spreading, and Type I collagen (Col-1) synthesis in HGFs were observed via Cell Counting Kit-8 (CCK-8) cell proliferation assay, Acridine Orange/Ethidium Bromide (AO/EB) fluorescence staining, SEM, and AFM. The modified coatings could significantly enhance the adhesive, proliferative, and spreading capacities of HGFs compared to the pure Ti substrate. The level of cell-secreted Col-1 rose alongside increasing doping concentration of Y and duration of cell cultivation (days) with a positive dependence between concentration and time.the surface of coatings with higher doping concentrations of Y harbored significantly reduced number of adherent S. mutans.Y-doped HA coatings could alter the surface properties of pure Ti with good biocompatibility and significant antibacterial properties. This surface modification method has significant application potential for improving the repair, regeneration, and integration of soft tissues on the surface of pure Ti.