真菌漆酶对两种活性染料的脱色研究

Enzymatic decolorization of textile dyes is a hot topic in the environmental biotechnology. In this study, laccases were prepared from three fungal strains of different genera and used to decolorize two active textile dyes, DyStar Blue Levafix CA and Ciba Blue FN-R. The optimal pH and temperature in the reaction system were pH4.0 and 40oC, respectively. The decolorization efficiencies of the the two dyes were slightly different in terms of individual laccases used, and decresed at a high dye content of above 5.0g/L. Nevertheless, all of the three laccases showed the power to efficiently decolorize the two dyes. Under the optimal reaction conditions, dyes of 0.1 g/L were completely decolorized by 1 U/L Panus rudis laccase during six hours. Our studies indicates that fungal laccases have potential in the application of cleaning textile dye effluents.