aKR1上游启动子分离及其表达分析

nchors, a kind of important proteins mediating protein-protein interaction, is closely related to the regulation of plant growth and development as well as stresses responses. CaKR1 isolated from pepper leaves (Capsici annuune L.) is a member of the anchored proteins and involve in pepper response to biotic and abiotic stresses by previous study, but the underlying mechanism is not well understood. In the present study, the -1594 bp upstream promoter sequence of the CaKR1 was isolated by genome walking technology and named CaKR1p and cis-elements such as SARE, ABRE, LTRE were found in this promoter. The promoter was fused to the GUS reporter gene to generate expression vector, which was transformed into tobacco by Agrobacterium mediated method and transgenic plants was acquired and confirmed. With T1 transgenic lines, we analyzed the expression of CaKR1p against the treatment of exogenous hormone, and found that the expression of GUS reporter gene was induced significantly by exogenously applied ABA and SA, suggesting that CaKR1 may involve in pepper response to abiotic and biotic stresses regulated by signaling mediated by ABA and SA, respectively, and the inducible expression of CaKR1 is possibly conferred by the SA and ABA responsive elements in its promoters.