仙鹤草活性组分对胰岛素抵抗HepG2细胞葡萄糖代谢的调控

Studied on the effects of FC and TC of active ingredients from agrimony on glucose metabolism in IR-HepG2 cells . Methods: establish insulin resistance model which HepG2 cells were induced by high glucose.The uptake and consumption of glucose in HepG2 cells were measured by 2-NBDG fluorescence detection tracer and glucose oxidase method respectively to establish the stable HepG2 insulin resistance model. The 0.1-2.5μg / mL FC and TC treated insulin resistance cells for 24 h respectively and observed the effects of active components on the glucose uptake and glucose consumption of insulin resistant HepG2 cells . The expression of IRS-1 and JNK genes in insulin signaling pathway was detected by RT-PCR.The results showed that HepG2 cells had a significant reduction in glucose uptake and consumption (p <0.05) were incubated with 55 mM high glucose for 24 h, and the stable insulin resistance model of human hepatocellular carcinoma was successfully induced. The effects of FC and TC on the glucose consumption and uptake of HepG2 cells and the expression of IRS-1 and JNK were detected, and we found that FC and TC can effectively improve insulin resistance of intracellular.