梨休眠相关基因bZIP的克隆与分析
loning and Expresssion Analysis of dormancy-related gene bZIP from Pyrus
以黄花梨（Pyrus pyrifolia var. huanghua）的花芽为材料，通过同源克隆技术首次从梨花芽中分离出bZIP同源基因。该bZIP基因全长1843 bp，开放阅读框长度1227 bp，可编码408个氨基酸, 命名为PpbZIP，(GenBank注册号: KC951877)。通过蛋白序列比对，发现PpbZIP与同为蔷薇科的苹果bZIP同源性高达92%。经过QRT-PCR发现，该基因在梨花芽从休眠到休眠解除后的表达量为先升高后降低，因此推到该基因可能与梨花芽休眠存在一定关系。
bZIP homology gene was isolated from the flower bud of Pyrus pyrifolia var. Huanghua by homology-based cloneing technique. The full length of the gene was 1843 bp with an open reading frame 1227 bp, while it can encodes 408 amino acids, named PpbZIP(NCBI number: KC951877). Though the analysis of protein, we found the PpbZIP gene had a high homology(92%) to the Malus x domestica. The bZIP gene showed an expression increasing at the initiate stage of endo-dormancy and then dropped until the total release of dormancy. It was speculated that the bZIP gene had a relationship with dormancy of pear flower bud.
吴少华、李永裕、宋燕春、黄添毅、陈铁娇
分子生物学植物学
梨休眠bZIPRACEQRT-PCR
PyrusdormancybZIPRACEQRT-PCR
吴少华,李永裕,宋燕春,黄添毅,陈铁娇.梨休眠相关基因bZIP的克隆与分析[EB/OL].(2013-05-14)[2025-06-29].http://www.paper.edu.cn/releasepaper/content/201305-183.点此复制
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