抗凝剂引起的蛋白变化可以在尿中更敏感的监测

Biomarker studies commonly monitor the composition of plasma, which is under strict homeostatic control, while urine is not. Urine is a partial filtrate of blood; however, its composition reflects systemic physiology. Here we show that changes in the coagulation status of blood can be more sensitively detected in urine than in plasma. Plasma and urine protein composition was analysed by LC-MS/MS and Western blot in six SD female rats before and after treatment with heparin or argatroban. LC-MS/MS and Western blot analyses demonstrated drug-induced increases in transferrin and hemopexin levels in urine but not in plasma. Our data indicates that urine may serve as a source for more sensitive detection of protein biomarkers than plasma.