SCP基因的柱头特异表达载体构建及拟南芥的遗传转化
onstruction of Expression Vector of senescence-associated cysteine protease SCP and Genetic Transformation in Arabidopsis thaliana
利用柱头特异表达的SLR启动子构建了SCP基因的柱头特异表达载体，酶切鉴定结果表明SCP载体构建成功，采用电转法转入农杆菌GV3101菌株，通过花序侵染法将载体进行拟南芥遗传转化，经基因组 PCR 鉴定表明特异表达载体已成功整合到拟南芥基因组中。激光共聚焦显微镜观察花柱头发现绿色荧光蛋白在柱头有强烈的表达，荧光显微分析显示BcSCP转基因拟南芥对花粉管萌发和延伸无影响。
We used the stigma-specific SLR1 promoter to construct SCP expression vector, digestion results showed that SCP vector was successfully constructed, the construct was then mobilized into Agrobacterium GV3101, followed by Agrobacterium-mediated transformation of wild-type Arabidopsis plants using the floral dip method (Clough and Bent, 1998), PCR identification showed that the carrier has been successfully integrated into the Arabidopsis genome. Laser scanning confocal microscopy was used to observe the expression of green fluorescent protein in the stigma. The fluorescence microscopic analysis showedConstruction of Expression Vector of senescence-associated cysteine protease SCP and Genetic Transformation in Arabidopsis thaliana that transgenic Arabidopsis had no effect on the germination and elongation of pollen tubes.
黄天虹、王成、李英、文锴、侯喜林
遗传学分子生物学植物学
蔬菜学BcSCP基因载体构建转基因拟南芥柱头特异表达花粉管生长
OlericultureBcSCP genevector constructiontransgenic Arabidopsisstigma-specific expressionelongation of pollen tubes
黄天虹,王成,李英,文锴,侯喜林.SCP基因的柱头特异表达载体构建及拟南芥的遗传转化[EB/OL].(2017-01-10)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201701-133.点此复制
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