同型半胱氨酸对卵母细胞发育过程中组蛋白甲基化和乙酰化的影响

Objective: To understand the effect of homocysteine on histone modification during oocyte development. Method: In this paper the follicle culture system and method of oocyte maturated in vitro were used and the process of oocyte development and maturation was observed. By immunofluorescent staining the influence of HCY on distribution of methylated histone H3K4, H3K9, and acetylated histone H3K9 during early stage of oocyte development was observed. And with the approach of real time PCR, the expression level of two enzymes controlling histone acetylation, histone deacetylase(HDAC) and histone acetyltransferase (GCN5), in oocyte maturated in vitro was compared between group coculture with homocysteine and control. Results: The process of oocyte maturation was significantly inhibited by homocysteine. Though the distribution patterns of methylated histone H3K4, H3K9 and acetylated histone H3K9, the staining intensity was decreased by homocysteine.The chromatin reveals slightly decondensated. The influence of HCY on GCN5 expression was tiny, but the expression of HDAC in oocyte was significantly inhibited.Conclusion: The effect of homocysteine on histone methylation and acetylation was indeed present during oocyte development. This may be the good reason for the decline of nuclear chromosome stability caused by homocysteine.