拟南芥中一个盐害诱导的WRKY转录因子的上游互作蛋白筛选
Screening of Interaction Proteins of A Salt-inducible WRKY Transcription Factor in Arabidopsis
在前期的工作，我们发现拟南芥的WRKY41转录因子基因显著地受盐害处理诱导。为了探明WRKY41的作用机制，我们首先将WRKY41基因的编码区克隆到酵母转录活性分析载体，发现它是一个转录抑制子。继而，我们克隆了拟南芥中丝裂原活化蛋白激酶(MPK)基因家族的20个基因的cDNA序列，构建了酵母双杂交载体，检测了AtWRKY41与20个MPK之间的互作情况，结果发现它能与多个AtMPK蛋白激酶互作。
In a previous study, we identified that Arabidopsis WRKY transcription factor gene WRKY41 was highly induced by salt treatment in root. To explore possible mechanism of it, we firstly cloned the coding region of WRKY41 into yeast vector and assayed its transcriptional activity. Our result showed AtWRKY41 is a transcriptional repressor. Further, we succeeded in cloning 20 mitogen-activated protein kinase (MPK) genes from wild-type Arabidopsis through RT-PCR, followed by yeast two-hybrid (Y2H) vector construction. Then a systematical Y2H screening of interactions between AtWRKY41 transcription factor and 20 MPKs was performed and it revealed AtWRKY41 interacted with quite a few AtMPKs.
王倩楠、赵新杰、江元清、牛芳芳
分子生物学植物学
拟南芥WRKY转录因子MPK互作模式
rabidopsisWRKYtranscription factorMPKinteraction pattern
王倩楠,赵新杰,江元清,牛芳芳.拟南芥中一个盐害诱导的WRKY转录因子的上游互作蛋白筛选[EB/OL].(2016-06-03)[2025-04-30].http://www.paper.edu.cn/releasepaper/content/201606-271.点此复制
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