The landscape of antibiotic resistance genes in the nasopharynx of preterm infants: Prolonged signature of hospitalization and effects by antibiotics
The landscape of antibiotic resistance genes in the nasopharynx of preterm infants: Prolonged signature of hospitalization and effects by antibiotics
ABSTRACT Respiratory pathogens, commonly colonizing nasopharynx, are among the leading causes of death due to antimicrobial resistance. Yet, antibiotic resistance determinants within nasopharyngeal microbial communities remain poorly understood. Utilizing shotgun metagenomics, we investigated the nasopharynx resistome development in preterm infants, assessed early antibiotic impact on its trajectory, and explored its association with clinical covariates. Our findings revealed widespread nasopharyngeal carriage of antibiotic resistance genes (ARGs) with resistomes undergoing transient changes, including increased ARG diversity, abundance, and composition alterations due to early antibiotic exposure. ARGs associated with the critical nosocomial pathogen Serratia marcescens persisted up to 8-10 months of age, representing a long-lasting hospitalization signature. The nasopharyngeal resistome strongly correlated with microbiome composition, with inter-individual differences and postnatal age explaining most of the variation. Our report on the collateral effects of antibiotics and prolonged hospitalization underscores the urgency of further studies focused on this relatively unexplored reservoir of pathogens and ARGs.
Dahle Ulf、Petersen Fernanda Cristina、Fugelseth Drude、Berild Dag、Dhariwal Achal、Greisen Gorm、Haaland Kirsti、Rajar Polona、?mdal Heidi Aar?、Salvadori Gabriela、Saugstad Ola Didrik
Centre for Antimicrobial Resistance, Norwegian Institute of Public HealthInstitute of Oral Biology, Faculty of Dentistry, University of OsloDepartment of Neonatal Intensive Care, Division of Pediatric and Adolescent Medicine, Oslo University Hospital Ullev?l||Institute of Clinical Medicine, Faculty of Medicine, Oslo UniversityDepartment of Infectious Diseases, Oslo University Hospital||Institute of Clinical Medicine, Faculty of Medicine, Oslo UniversityInstitute of Oral Biology, Faculty of Dentistry, University of OsloDepartment of Neonatology, Copenhagen University Hospital RigshospitaletDepartment of Neonatal Intensive Care, Division of Pediatric and Adolescent Medicine, Oslo University Hospital Ullev?lInstitute of Oral Biology, Faculty of Dentistry, University of Oslo||Department of Neonatal Intensive Care, Division of Pediatric and Adolescent Medicine, Oslo University Hospital Ullev?lInstitute of Oral Biology, Faculty of Dentistry, University of OsloInstitute of Oral Biology, Faculty of Dentistry, University of OsloDepartment of Pediatric Research, Oslo University Hospital, University of Oslo
医学研究方法微生物学儿科学
respiratory resistomemetagenomicsnasopharynxmicrobiomepreterm infantsantibioticsantimicrobial resistance
Dahle Ulf,Petersen Fernanda Cristina,Fugelseth Drude,Berild Dag,Dhariwal Achal,Greisen Gorm,Haaland Kirsti,Rajar Polona,?mdal Heidi Aar?,Salvadori Gabriela,Saugstad Ola Didrik.The landscape of antibiotic resistance genes in the nasopharynx of preterm infants: Prolonged signature of hospitalization and effects by antibiotics[EB/OL].(2025-03-28)[2025-05-22].https://www.biorxiv.org/content/10.1101/2023.08.09.552435.点此复制
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