S100A9介导食管鳞癌细胞辐射抵抗的初步研究

Objective: To investigate the effects of S100A9 in regulating the radiosensitivity of esophageal squamous carcinoma (ESCC) cells. Methods: The S100A9 overexpressed or silenced human esophageal squamous carcinoma cell model were constructed using TE-1 and KYSE-30 cell lines, and the clonogenic assay was used to determine the effect of S100A9 on the radiosensitivity of TE-1 and KYSE-30 cells. By applying flow cytometry analysis, the influence of S100A9 in regulating irradiation (IR) -induced apoptosis was assayed in vitro. Transwell assay was applied to detect the effect of S100A9 on migration of ESCC cells. Western Blot and immunofluorescence staining were performed to identify the expression of DNA damage related protein γ-H2AX. Results: Overexpressed S100A9 could increase the clone formation ability and migration of TE-1 cells, while reduce the apoptosis and the γ-H2AX expression after IR exposure, which indicated that S100A9 could promote the radioresistance of ESCC cells. And vice versa. Conclusions: S100A9 might play a crucial role in regulating the radiosensitivity of ESCC, with the mechanism through regulating IR-induced apoptosis and DNA damage of ESCC cells. Nevertheless, the specific molecular mechanisms need further in-detailed investigation.