中枢肿瘤坏死因子-α对糖尿病大鼠交感神经活动的影响
Brain tumour necrosis factor-α contributes to sympathoexcitation in diabetic rats
本研究旨在探讨中枢肿瘤坏死因子-α（TNF-α）与其合成阻断剂己酮可可碱（PTX）对糖尿病（DM）大鼠交感神经活动的影响。本试验通过将健康雄性SD大鼠随机分为糖尿病干预组（DM＋PTX）、糖尿病对照组（DM+VEH）、正常干预组（SHAM＋PTX）和正常对照组（SHAM+VEH）,并采用高脂高糖饲料喂养4周加链脲佐菌素注射建立糖尿病大鼠模型，干预组通过侧脑室插管给予PTX，对照组给予人工脑脊液（VEH）。4周后，对各组大鼠分别进行体重、血糖测量。应用免疫组化检测大鼠中枢下丘脑室旁核（PVN）内TNF-α表达，并运用电极记录肾交感神经放电情况。所得结果与SHAM组比较，（1） DM组大鼠PVN内TNF-α表达增加，且DM+SHAM组高于DM+PTX组；（2） DM组大鼠肾交感神经放电明显增强；DM+PTX组与DM+SHAM组比较，肾交感神经放电减弱。阻断中枢PVN区TNF-α合成可以明显降低糖尿病时交感神经兴奋性，最终初步证明中枢TNF-α对糖尿病大鼠交感神经活动存在着重要的影响。
he present study was undertaken to investigate the effect of brain TNF-α on the sympathetic nerve activity in diabetic rats. Sprage-Dawley rats (200±30g) were fed with high fat and sugar diet for 4 weeks following by injection of streptozotocin to induce the diabetes mellitus. Rats were implanted with paraventricular nucleus (PVN) cannulae and treated with the cytokine synthesis inhibitor pentoxifylline (PTX, 10 ug/h), or vehicle (VEH) for 4 weeks. Rats were randomly divided into SHAM+VEH, SHAM+PTX, DM+VEH, and DM+PTX groups. Immunohistochemisty were used to detect the expression of TNF-α in the PVN of hypothalamus, renal sympathetic nerve activity (RSNA) was recorded. Compared with sham groups, diabetic rats had higher level of TNF-α in the PVN. ICV treatment with PTX attenuated TNF-α expression in the PVN compared with VEH-treated diabetic rats. Compared with sham groups, diabetic rats had augmented renal sympathetic nerve activity. In contrast, ICV treatment with PTX decreased sympathetic nerve activity. In conclusion, TNF-α in the PVN contributes to sympathoexcitation in diabetes mellitus.
康玉明、齐杰、任晓霞、苏青、许艳艳、宋新爱
基础医学生理学神经病学、精神病学
交感神经肿瘤坏死因子-α糖尿病下丘脑室旁核
sympathetic nerveTNF-αdiabete mellitusparaventricular nucleus of hypothalamus
康玉明,齐杰,任晓霞,苏青,许艳艳,宋新爱.中枢肿瘤坏死因子-α对糖尿病大鼠交感神经活动的影响[EB/OL].(2011-08-11)[2025-08-06].http://www.paper.edu.cn/releasepaper/content/201108-188.点此复制
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