Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation
Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation
ABSTRACT Biological and biochemical functions of immunity transcription factor NF-κB in basal metazoans are largely unknown. Herein, we characterize transcription factor NF-κB from the demosponge Amphimedon queenslandica (Aq), in the phylum Porifera. Structurally and phylogenetically, the Aq-NF-κB protein is most similar to NF-κB p100 and p105 among vertebrate proteins, with an N-terminal DNA-binding/dimerization domain, a C-terminal Ankyrin (ANK) repeat domain, and a DNA binding-site profile more similar to human NF-κB proteins than Rel proteins. Aq-NF-κB also resembles the mammalian NF-κB protein p100 in that C-terminal truncation results in translocation of Aq-NF-κB to the nucleus and increases its transcriptional activation activity. Overexpression of a human or sea anemone IκB kinase (IKK) can induce C-terminal processing of Aq-NF-κB in vivo, and this processing requires C-terminal serine residues in Aq-NF-κB. Unlike human NF-κB p100, however, the C-terminal sequences of Aq-NF-κB do not effectively inhibit its DNA-binding activity when expressed in human cells. Tissue of another demosponge, a black encrusting sponge, contains NF-κB site DNA-binding activity and an NF-κB protein that appears mostly processed and in the nucleus of cells. NF-κB DNA-binding activity and processing is increased by treatment of sponge tissue with LPS. By transcriptomic analysis of A. queenslandica we identified likely homologs to many upstream NF-κB pathway components. These results present a functional characterization of the most ancient metazoan NF-κB protein to date, and show that many characteristics of mammalian NF-κB are conserved in sponge NF-κB, but the mechanism by which NF-κB functions and is regulated in the sponge may be somewhat different.
Nambiar Avanti、Patel Devansh、Yapo Alia、Williams Leah M.、Mansfield Katelyn M.、Rasmussen Anna、Afghani Jamie、Babaei Milad、Babaei Mohammad、Bonilla Arianna、Carpenter Sheldon、Christie Blake、Huang Xinli、Kasabwala Aarti、Kehoe Leanne、LeVan Andre、Liu Yuekun、Murphy Emily、Esau Lindsie、Gilmore Thomas D.、Chen Tiffany、Sanchez Selma、Dam Katie、Inge Melissa M.、Hanchate Anvita、Cyr Andrew、Olive Meagan、Albert Colleen、Dulock Nicholas、Stejskal Camilla、Esonwune Stephanie、Browne Amanda、Pavesi Flaminio、Samofalova Yelena、Siggers Trevor、Kobayashi Ryan、Jennings Timothy、Tang Yinian、Bagdasaryants Abigail、Yunes Sarah A.、Agrba Mikhail、Petty Christopher A.、Cleary John P.、Lee Migi、Andersson Cecilia、Erdene Galbadrakh
Molecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityDepartment of Biology, Boston UniversityDepartment of Biology, Boston UniversityDepartment of Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityDepartment of Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityDepartment of Biology, Boston University||Molecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityDepartment of Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston UniversityMolecular Biology Laboratory (BB522) of the Program in Biochemistry & Molecular Biology, Boston University
分子生物学细胞生物学遗传学
NF-κB transcription factorevolutionlipopolysaccharidesignal transductionimmunologyspongeAmphimedon queenslandica
Nambiar Avanti,Patel Devansh,Yapo Alia,Williams Leah M.,Mansfield Katelyn M.,Rasmussen Anna,Afghani Jamie,Babaei Milad,Babaei Mohammad,Bonilla Arianna,Carpenter Sheldon,Christie Blake,Huang Xinli,Kasabwala Aarti,Kehoe Leanne,LeVan Andre,Liu Yuekun,Murphy Emily,Esau Lindsie,Gilmore Thomas D.,Chen Tiffany,Sanchez Selma,Dam Katie,Inge Melissa M.,Hanchate Anvita,Cyr Andrew,Olive Meagan,Albert Colleen,Dulock Nicholas,Stejskal Camilla,Esonwune Stephanie,Browne Amanda,Pavesi Flaminio,Samofalova Yelena,Siggers Trevor,Kobayashi Ryan,Jennings Timothy,Tang Yinian,Bagdasaryants Abigail,Yunes Sarah A.,Agrba Mikhail,Petty Christopher A.,Cleary John P.,Lee Migi,Andersson Cecilia,Erdene Galbadrakh.Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation[EB/OL].(2025-03-28)[2025-07-01].https://www.biorxiv.org/content/10.1101/691097.点此复制
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